JOURNAL ARTICLE

[Epidemiological and molecular characteristics of hospital-acquired methicillin-resistant Staphylococcus aureus strains isolated in Hacettepe University Adult Hospital in 2004-2005]

Hadim Akoğlu, Pınar Zarakolu, Belgin Altun, Serhat Ünal
Mikrobiyoloji Bülteni 2010, 44 (3): 343-55
21063984
The aim of this study was to determine the epidemiological and molecular characteristics of hospital-acquired (HA)-methicillin-resistant Staphylococcus aureus (MRSA) isolates by investigating the distribution of clinical samples according to the hospital wards, antibiotic susceptibility patterns, staphylococcal chromosome cassette mec (SCCmec) types and the presence of Panton-Valentine Leukocidin (PVL) genes. A total of 110 MRSA isolates obtained from various clinical samples of inpatients at Hacettepe University Adult Hospital between January 2004 and December 2005 were included in the study. The identification of the isolates was done by BD Sceptor automated system (Becton Dickinson, USA). The mecA gene, SCCmec types and PVL genes were detected by polymerase chain reaction (PCR). Pulsed field gel electrophoresis (PFGE) was performed to examine the clonal relatedness. The susceptibility testing was performed for some antibiotics by E-test (AB Biodisk, Sweden) and for the others by disk diffusion methods according to the Clinical and Laboratory Standards Institute (CLSI) recommendations. The clinical samples (35 blood, 37 pus, 23 deep tracheal aspiration, 5 catheter, and 10 other samples) that yielded the MRSA strains were isolated from patients (71.5%) at intensive care units and surgical wards. All the isolates were positive for mecA gene. Of the isolates, 68 (61.8%) were harboring SCCmec type III, 38 (34.5%) SCCmec variant IIIB, and 3 (2.7%) SCCmec type IV. One isolate which was mecA gene positive could not be classified in any of the SCCmec types. PVL was positive in 14 (12.7%) of the isolates. All MRSA strains were susceptible to tigecycline, linezolid, vancomycin and teicoplanin; however, exhibited high rates (> 90%) of resistance to gentamicin, ciprofloxacin and rifampin. Susceptibility rates to trimethoprim/sulfamethoxazole was 90%, clindamycin 53% and erythromycin 32%. Eight pulsotypes were distinguished on the basis of PFGE (A, B, C, D, K, L, N, O). Of the total isolates, 92.7% belonged to pulsotype A. HA-MRSA strains predominantly isolated from pus and blood samples of inpatients at intensive care units and surgical wards in our hospital were multi-resistant. Majority of these isolates were SCCmec III, or variant IIIB type. Although PVL is known as a common virulence factor of community-acquired MRSA, HA-MRSA isolates in our center have a considerable rate of PVL positivity pointing out the importance of surveillance of the changing epidemiology of MRSA.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
21063984
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"