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Quantitative detection of enterotoxigenic Bacteroides fragilis subtypes isolated from children with and without diarrhea.

V R C Merino, V Nakano, C Liu, Y Song, S M Finegold, M J Avila-Campos
Journal of Clinical Microbiology 2011 January
A rapid real-time PCR (RT-PCR) approach was developed to detect the bft gene subtypes in Bacteroides fragilis isolated from fecal samples. DNA obtained from diarrhea (110) and nondiarrhea (150) samples was evaluated. Subtype 1 was observed in 9 (8.2%) diarrhea and 7 (4.7%) nondiarrhea samples. Subtype 2 was not detected in any DNA samples, and subtype 3 was observed in only 1 diarrhea sample. The presence of the bft-1 gene did not show any statistically significant differences between the groups of children. This technique could be used to evaluate a possible correlation between disease and the presence of B. fragilis enterotoxin.

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