Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor

Ken'ichiro Matsumoto, Kei Kitagawa, Sung-Jin Jo, Yuyang Song, Seiichi Taguchi
Journal of Biotechnology 2011 April 10, 152 (4): 144-6
Lipopolysaccharides free P[3-hydroxybutyrate (3HB)-co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3HV unit accumulated 8-47wt% of P(3HB-co-3HV). The 3HV fraction in the copolymer was varied from 0 to 28mol% depending on the propionate concentrations.

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