Temporal and spatial expression of TGF-beta1 in an Achilles tendon section model after application of platelet-rich plasma

Dimitrios N Lyras, Konstantinos Kazakos, Marios Tryfonidis, George Agrogiannis, Sotirios Botaitis, Anna Kokka, George Drosos, Konstantinos Tilkeridis, Dionysios Verettas
Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons 2010, 16 (3): 137-41

BACKGROUND: To investigate the effect of platelet-rich plasma (PRP) on TGF-beta1 expression during tendon healing.

METHODS: We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-beta primary antibody were performed.

RESULTS: The pattern of expression of TGF-beta1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls.

CONCLUSIONS: Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-beta1.


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