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EVALUATION STUDIES
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Self-assembly-peptide hydrogels as tissue-engineering scaffolds for three-dimensional culture of chondrocytes in vitro.
Macromolecular Bioscience 2010 October 9
The promising potential of a RAD-16 self-assembly-peptide hydrogel as a scaffold for tissue-engineered cartilage was investigated. Within 3 weeks of in vitro culture, chondrocytes within the hydrogel produced a high amount of GAG and type-II collagen, which are the components of cartilage-specific extracellular matrix (ECM). With the culture time increased, toluidine-blue staining for GAG and immuno-histochemistry staining for type-II collagen of the chondrocytes-hydrogel composites became more intense. Analysis of the gene expression of the ECM molecules also confirmed the chondrocytes in the peptide hydrogel maintained their phenotype within 3 weeks of in vitro culture.
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