OPEN IN READ APP
JOURNAL ARTICLE

A strawberry notch homolog, let-765/nsh-1, positively regulates lin-3/egf expression to promote RAS-dependent vulval induction in C. elegans

Carrie L Simms, David L Baillie
Developmental Biology 2010 May 15, 341 (2): 472-85
20230814
The specification and patterning of vulval precursor cells (VPCs) in Caenorhabditiselegans is achieved using a conserved EGFR/RAS signaling pathway that is activated by the ligand lin-3/EGF, which is secreted by the neighboring somatic gonad. Previous work has demonstrated that the expression of lin-3 must be tightly regulated to ensure that only three of six equivalent VPCs are induced to differentiate into the mature vulva. Here, we have identified a novel regulator of EGFR/RAS signaling, let-765/nsh-1, that functions upstream of the pathway to promote vulval induction. let-765 encodes a conserved DExD/H box helicase protein and is the C. elegans ortholog of Drosophila strawberry notch. By investigating genetic interactions between let-765 and RAS pathway genes as well as with synthetic multivulva (synMuv) genes, we have demonstrated that let-765 positively regulates the RAS pathway and antagonizes synMuv activity at the level of lin-3/EGF. In support of these proposals, we found that LET-765 is required for producing wild-type levels of lin-3 mRNA. Mutations in let-765 result in pleiotropic phenotypes that imply its function must be required in multiple developmental processes and, together with data presented here, suggest that LET-765 promotes the expression of diverse targets, potentially through interactions with transcriptional activator or repressor complexes.

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Available on the App Store

Available on the Play Store
Remove bar
Read by QxMD icon Read
20230814
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"