JOURNAL ARTICLE

High-affinity immobilization of proteins using biotin- and GST-based coupling strategies

Stephanie Q Hutsell, Randall J Kimple, David P Siderovski, Francis S Willard, Adam J Kimple
Methods in Molecular Biology 2010, 627: 75-90
20217614
Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.

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