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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.
Lab on a Chip 2009 December 8
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.
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