Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device

Kirsty J Shaw, Domino A Joyce, Peter T Docker, Charlotte E Dyer, John Greenman, Gillian M Greenway, Stephen J Haswell
Lab on a Chip 2009 December 7, 9 (23): 3430-2
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.

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