[Biological effects of lipopolysaccharide, transforming growth factor-beta1 on murine bone marrow-derived dendritic cells].

AIM: To explore method of stimulating murine bone marrow-derived dendritic cells by lipopolysaccharide (LPS), transforming growth factor-beta1 (TGF-beta1) and to study their biological character.

METHODS: Murine bone marrow-derived dendritic cells were cultivated with cytokine GM-CSF and IL-4 for 6 days, BMDC was stimulated by control, LPS, TGF-beta1, LPS +TGF-beta1 for 48 hours respectively.Morphological characters of BMDC were observed by a inversed microscope, surface molecules such as CD(11C), CD(80), CD(86) and MHC II were detected by flowcytometry, Interleukin-6 and interleukin-12 p70 in co-culture medium was quantified by ELISA.

RESULTS: In LPS group it presented the most typical DC morphology with the highest expression of CD(80), CD(86) and MHC II, the strongest ability in mixed lymphocyte reaction, higher level of IL-6 and IL-12 p70 compared with control, TGF-beta1, LPS+TGF-beta1 (P<0.05). While in TGF-beta1 group it presented the less typical DC morphology with the lower expression of CD(80), CD(86), MHC II, weaker ability in mixed lymphocyte reaction, and lower levels of IL-6 and IL-12 p70 compared with control and LPS (P<0.05).

CONCLUSION: LPS can stimulate maturation of BMDC in its late differentiation which makes it presents a more significant biological characteristics.TGF-beta1 can inhibit maturation but not differentiation of BMDC thereby can prevent its biological characteristic presentation.