The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths

Kirsty J Shaw, Lauren Thain, Peter T Docker, Charlotte E Dyer, John Greenman, Gillian M Greenway, Stephen J Haswell
Analytica Chimica Acta 2009 October 12, 652 (1): 231-3
DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25ng) compared to the absence of RNA (5ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications.

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