Quantitation of catalpol in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its pharmacokinetic study

Rong Lu, Yuan Gu, Duanyun Si, Changxiao Liu
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 2009 November 1, 877 (29): 3589-94
A sensitive, rapid and specific liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay has been established for the quantitation of catalpol in rat plasma. Plasma samples were treated by precipitating protein with methanol and were chromatographed by a Diamonsil C(18) column (150 mm x 4.6 mm I.D., 5 microm) with the mobile phase consisting of methanol and 10 mM ammonium formate (30:70, v/v). The selected reaction monitoring (SRM) transitions were performed at m/z 380.1-->183.0 for catalpol and m/z 364.3-->167.0 for aucubin (IS) in the positive ion mode with electrospray ionization (ESI) source. Calibration curve was linear over the concentration range of 10-20,000 ng/mL. The mean recovery was 76.5+/-5.2% and the matrix effect ranged from -5.1 to 13.0%. The intra- and inter-day precisions were less than 6.3 and 14.6%, respectively, and the accuracy was within +/-5.6%. Catalpol was stable in possible conditions of storing and handling. The validated method has been successfully applied to determine the plasma concentration of catalpol for a pharmacokinetic study of catalpol after oral administration of 50 mg/kg to rats.

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