[Killer immunoglobulin-like receptor gene genotype and expression pattern in NK-92MI and K562 cells]

Li-ping Dou, Wan-ming DA, Jing-hua Liu, Jing-fen Sun, Yu Zhao, Quan-shun Wang, Chun-ji Gao, Li Yu
Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology 2009, 25 (9): 767-9

AIM: To make clear the repertoire of Killer immunoglobulin-like receptor at the level of DNA and RNA in NK-92MI and K562 cells and compare KIRs gene genotype and expression patterns in both cells.

METHODS: KIRs gene genotype, include KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 3DL1, 3DL2, 3DL3, 3DS1, 2DP1, 3DP1, was analyzed by PCR. KIRs gene phenotype, include KIR2DL1, KIR2DL2, KIR2DL3, KIR3DL1, KIR2DS1, KIR2DS3, KIR2DS2/4 gene, was determined by RT-PCR and sequencing.

RESULTS: KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5A, 2DS2, 2DS4*003-006, 2DS5, 3DL1, 3DL2 and 3DL3 gene were present in K562 cells, but all corresponding receptors were not expressed by K562 cells. KIR2DL1, 2DL2, 2DL3, 2DL4, 2DS2, 2DS4*001/002, 2DS4*003-006, 3DL1, 3DL2 and 3DL3 gene were present in NK-92MI cells, but only transcripts of KIR2DL1, KIR2DL3 and KIR2DS2/4 were detectable.

CONCLUSION: KIR2DL1, 2DL2, 2DL3, 2DL4, 2DS2, 2DS4*003-006, 3DL1, 3DL2 and 3DL3 gene were present in both K562 and NK-92MI cells. All corresponding KIR receptors were not expressed by K562 cells and only partial KIRs by NK-92MI cells. The repertoire of KIRs were specific for K562 and NK-92MI cells.

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