We have located links that may give you full text access.
EVALUATION STUDIES
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Establishment of an effective virus induced gene silencing system with BSMV in Haynaldia villosa.
Molecular Biology Reports 2010 Februrary
In recent years, virus-induced gene-silencing (VIGS) has shown to be a powerful reverse genetics tool for gene function study. In this paper, an effective and persistent virus-induced gene silencing (VIGS) system was established with barley stripe mosaic virus (BSMV) for Haynaldia villosa. Examination of GFP gene expression showed that the BSMV vector moved systemically from leaf to leaf in inoculated H. villosa plants. This vector, expressing a cDNA fragment of phytoene desaturase (PDS), suppressed the transcript level of endogenous Hv-PDS gene as early as 6 days after inoculation, and caused photobleaching symptoms mainly on the newly developed upper leaves. Moreover, PDS gene silencing phenotype persisted through the whole growing period in H. villosa. With this established VIGS system, function analysis of a powdery mildew resistance related gene Hv-LRR was successfully performed. This is the first report that BSMV can be used for VIGS in a wild relative species of wheat. The established VIGS system will be a powerful reverse genetics tool for gene function study in H. villosa, an important genetic resource for wheat breeding.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app