JOURNAL ARTICLE

Cytogenetic analysis of Populus trichocarpa—ribosomal DNA, telomere repeat sequence, and marker-selected BACs

M N Islam-Faridi, C D Nelson, S P DiFazio, L E Gunter, G A Tuskan
Cytogenetic and Genome Research 2009, 125 (1): 74-80
19617699
The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

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