JOURNAL ARTICLE

Stress-related hormones modulate cytokine expression in the head kidney of gilthead seabream (Sparus aurata)

Juan Castillo, Mariana Teles, Simon Mackenzie, Lluis Tort
Fish & Shellfish Immunology 2009, 27 (3): 493-9
19591943
Neuro-endocrine and immune systems closely interact in fish, and their regulation is crucial for the maintenance of good health of cultured fish. We have used the seabream head kidney to study whether stress-related hormones can modulate the immune response. For this purpose, the effects of adrenaline, adrenocorticotropic hormone (ACTH) and cortisol on the expression of pro-inflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and the anti-inflammatory cytokine TGF-beta1 were determined by means of quantitative real-time PCR on isolated head kidney cells. ACTH (150 ng mL(-1)) caused an acute increase of TNF-alpha and IL-6 mRNA levels as well as an inhibition of IL-1beta expression. The expression of the anti-inflammatory cytokine TGF-beta1 was also increased, although in a lower extent. Adrenaline (1 muM) early effects were only clear inhibiting IL-1beta expression but not TNF-alpha, IL-6 or TGF-beta1 mRNA levels, while a longer exposure to the hormone inhibited all cytokines. Moreover, cortisol (50 and 100 ng mL(-1)) reduced the expression of all cytokines in a dose-dependent manner. Bacterial lipopolysaccharide (LPS) stimulated IL-1beta expression and inhibited that of the anti-inflammatory TGF-beta1, although it was ineffective on TNF-alpha and IL-6. In addition, adrenaline and cortisol decreased the LPS-stimulated IL-1beta expression, further demonstrating their previously reported anti-inflammatory effects. The combination of ACTH and LPS, on the other hand, did not affect LPS-stimulated IL-1beta expression but was effective increasing TNF-alpha expression. Taking all these results in consideration, we conclude that the expression of pro- and anti-inflammatory cytokines in the seabream head kidney is highly influenced by stress-related hormones, thus indicating an important role for the endocrine system in the modulation of the immune response in teleost fish.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
19591943
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"