We have located links that may give you full text access.
Journal Article
Research Support, Non-U.S. Gov't
The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells.
Journal of Endodontics 2009 June
INTRODUCTION: The current study investigated the effectiveness of enamel matrix derivative (EMD) and mineral trioxide aggregate (MTA) in inducing the differentiation of human dental pulp cells (HDPCs) into odontoblast-like cells in vitro.
METHODS: The effects of MTA and EMD on odontoblastic differentiation were indexed by alkaline phosphatase (ALP) activity and the expression of odontoblastic/osteoblastic markers, as determined by reverse-transcription polymerase chain reaction analysis. Mineralization was also evaluated by the staining of calcium deposits with Alizarin red. Cells were treated with a combination of MTA and EMD or with MTA alone.
RESULTS: Compared with MTA-treated cells on day 3, MTA/EMD-treated cells exhibited significantly greater increases in ALP activity and in dentin sialophosphoprotein and bone sialoprotein expression. Mineralization was significantly greater on day 7 in MTA/EMD-treated cells than in MTA-treated cells.
CONCLUSIONS: These results suggest that a combination of MTA and EMD promotes more rapid differentiation in HDPCs than MTA alone and that this combination is thus a potential pulp-capping agent.
METHODS: The effects of MTA and EMD on odontoblastic differentiation were indexed by alkaline phosphatase (ALP) activity and the expression of odontoblastic/osteoblastic markers, as determined by reverse-transcription polymerase chain reaction analysis. Mineralization was also evaluated by the staining of calcium deposits with Alizarin red. Cells were treated with a combination of MTA and EMD or with MTA alone.
RESULTS: Compared with MTA-treated cells on day 3, MTA/EMD-treated cells exhibited significantly greater increases in ALP activity and in dentin sialophosphoprotein and bone sialoprotein expression. Mineralization was significantly greater on day 7 in MTA/EMD-treated cells than in MTA-treated cells.
CONCLUSIONS: These results suggest that a combination of MTA and EMD promotes more rapid differentiation in HDPCs than MTA alone and that this combination is thus a potential pulp-capping agent.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app