Platelet-rich plasma and platelet-rich fibrin in human cell culture

Volker L W Gassling, Yahya Açil, Ingo N Springer, Nina Hubert, Jörg Wiltfang
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics 2009, 108 (1): 48-55

OBJECTIVES: The clinical use of platelet-rich plasma (PRP) for preprosthetic surgery has been a matter of controversy until now. Only recently, a new blood preparation has been developed which results in platelet-rich fibrin (PRF). The objective of the present investigation was to examine the growth factor release from PRP and PRF in vitro.

STUDY DESIGN: Whole blood samples from healthy participants (n = 10) were drawn to generate PRP and PRF. Human osteoblasts (O), human fibroblasts (F), and human osteoblast-derived osteosarcoma cells (Saos-2) were used for the cell culture. Cells of each cell line were cultivated, and PRP- or PRF-preparations added for ten days. The drawn medium was pooled and the quantities of growth factors (platelet-derived growth factor isomers AB and BB, insulin-like growth factor I, and transforming growth factor (TGF) isomers beta1 and beta2) analyzed by enzyme-linked immunosorbent assay.

RESULTS: In osteoblast and Saos-2 cultures, cytokine concentrations were significantly higher for PRP than for PRF (P < .05). In fibroblast cultures, results were the same with the exception of TGF-beta2 (P < .05).

CONCLUSIONS: This study demonstrates that PRP application in cell cultures leads to higher levels of growth factors than PRF application.

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