JOURNAL ARTICLE

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

Ansgar Huebner, Dan Bratton, Graeme Whyte, Min Yang, Andrew J Demello, Chris Abell, Florian Hollfelder
Lab on a Chip 2009 March 7, 9 (5): 692-8
19224019
We describe the design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow. Aqueous picoliter droplets are trapped en masse and optically monitored for extended periods of time. Such an array-based approach is used to characterize droplet shrinkage, aggregation of encapsulated E. coli cells and enzymatic reactions. We also demonstrate that trapped droplets may be recovered from the microfluidic array for further processing.

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