JOURNAL ARTICLE

Chemical genetics reveal the novel transmembrane protein BIL4, which mediates plant cell elongation in brassinosteroid signaling

Ayumi Yamagami, Miki Nakazawa, Minami Matsui, Masafumi Tujimoto, Masaaki Sakuta, Tadao Asami, Takeshi Nakano
Bioscience, Biotechnology, and Biochemistry 2009, 73 (2): 415-21
19202280
Steroid hormones are conserved between animals and plants as signaling molecules to control growth and development. Plant steroid hormones, brassinosteroids (BRs), appear to play an important role in plant cell elongation. BRs bind to leucine-rich repeat kinase BRASSINOSTEROID-INSENSITIVE 1 (BRI1) localized to the plasma membrane, activate transcription factors in collaboration with cytosolic kinases and phosphatases, and regulate BR-responsive gene expression, but the details regarding the BR signaling pathway from perception to nuclear events remain unknown. In this study we used chemical genetics to identify an evolutionarily conserved transmembrane protein, Brz-insensitive-long hypocotyls 4 (BIL4), and demonstrated its role as a critical component of plant cell elongation occurring upon BR signaling. A dominant mutation, bil4-1D, showed cell elongation in the presence of the BR-specific inhibitor Brz. Brz suppresses expression of the BIL4 gene in wild-type plants, and overexpression of BIL4 in bil4-1D suppresses the BR deficiency caused by Brz. Our results indicate that BIL4 mediates cell elongation on BR signaling.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
19202280
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"