An ionic liquid supported CeO2 nanoshuttles-carbon nanotubes composite as a platform for impedance DNA hybridization sensing

Wei Zhang, Tao Yang, Xuming Zhuang, Zhiyan Guo, Kui Jiao
Biosensors & Bioelectronics 2009 April 15, 24 (8): 2417-22
A novel nanocomposite membrane, comprising of nanosized shuttle-shaped cerium oxide (CeO(2)), single-walled carbon nanotubes (SWNTs) and hydrophobic room temperature ionic liquid (RTIL) 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF(6)), was developed on the glassy carbon electrode (GCE) for electrochemical sensing of the immobilization and hybridization of DNA. The properties of the CeO(2)-SWNTs-BMIMPF(6)/GCE, the characteristics of the immobilization and hybridization of DNA were studied by cyclic voltammetry and electrochemical impedance spectroscopy (EIS) using [Fe(CN)(6)](3-/4-) as the redox indicator. The synergistic effect of nano-CeO(2), SWNTs and RTIL could dramatically enhance the sensitivity of DNA hybridization recognition. The electron transfer resistance (R(et)) of the electrode surface increased after the immobilization of probe ssDNA on the CeO(2)-SWNTs-BMIMPF(6) membrane and rose further after the hybridization of the probe ssDNA with its complementary sequence. The remarkable difference between the R(et) value at the probe DNA-immobilized electrode and that at the hybridized electrode could be used for label-free EIS detection of the target DNA. The sequence-specific DNA of phosphoenolpyruvate carboxylase (PEPCase) gene from transgenically modified rape was detected by this DNA electrochemical biosensor. Under optimal conditions, the dynamic range for detecting the sequence-specific DNA of the PEPCase gene was from 1.0x10(-12) mol/L to 1.0x10(-7) mol/L, and the detection limit was 2.3x10(-13) mol/L, suggesting that the CeO(2)-SWNTs-BMIMPF(6) nanocomposite hold great promises for the applications in sensitive electrochemical biosensor.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"