[An experimental study of rabbits' wound repair by amniotic carrier complex membrane containing bFGF and vitamin C and loaded with BMSCs].

OBJECTIVE: The amniotic carrier complex membrane, which contains bFGF and vitamin C (VitC) and is loaded with BMSCs, is planted into the deeply-partial wounds of rabbits. To explore its influence on the epidermis renascence and regenerating speed in the process of the dermis restore.

METHODS: BMSCs were isolated from the marrows of 24 healthy 3-month-old New Zealand rabbits, male or female, weighing 1.0-1.5 kg. The BMSCs were cultured in vitro and purified, and then amniotic carrier complex membrane was prepared, whose size was 4.52 cm2. Three deep-partial wounds, with the area of about 3.14 cm2, were produced on the back of each rabbit. All the wounds were randomly divided into 3 groups: group A, group B and group C. Group A was the experimental group in which the amniotic carrier complex membrane was planted, including 1 ml BMSCs, 10 mL bFGF (0.2 mg/L) and 10 mL VitC (0.02 g/L). In group B, the amniotic carrier complex membrane was planted, including only 1 mL BMSCs. In group C, the amniotic carrier complex membrane alone was planted. After the operation, general observation was conducted. At postoperative 7, 14 and 21 days, respectively, the observation by HE, Masson, Van Gieson staining and immunohistochemical staining of collagen type I was performed. The ink perfusion method was performed to evaluate the velocity and the quality of the wound healing after the transplantation.

RESULTS: All the wounds obtained good healing. At 14 days after the operation, the ratio of wound healing was 60%, 41% and 23% in groups A, B and C, respectively. At 21 days after the operation, the the ratio of wound healing was 99%, 90% and 81% in groups A, B and C, respectively. There were significant differences between any two groups (P < 0.05). The depth of the newborn dermis, the number of the active collagen type I masculine cells and the number of the blood vessels in group A were better and more than in group B. And those in group B were better and more than in group C. At the exterior area of the newborn dermis, there was lots of regenerated epidermis from the peripheral normal skin, which in group A was better than in group B, and in group B was better than in group C.

CONCLUSION: The amniotic carrier complex membrane transplanted to deep-partial wounds, which is appended with BMSCs, bFGF and VitC, can accelerate repair and reconstruction of the dermis. There has an optimal time of the renascence and regeneration of the epidermis in the process of dermis repair.