[Genotype analysis of enterovirus type 71 detected from patients with hand-foot-mouth disease in Shenzhen]

Ya-qing He, Hong Yang, Lin-lin Li, Jie Tan, Li Zhou, Li-sha Mao, Fan Yang, Jian-jun Liu, Xing Lv
Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi 2008, 29 (8): 790-3

OBJECTIVE: To carry out genotype analysis of enterovirus type 71, detected from hand-foot-mouth disease patients in Shenzhen in 2004.

METHODS: All samples were tested by reverse transcription-polymerase chain reaction (RT-PCR) using EV71 specific primer. The VP1 and VP4 of EV71 were cloned and sequenced. A phylogenetic tree was constructed by comparing the sequences with genogroups A, B and C genotypes using TreeView and PHYLIP software (3.6b).

RESULTS: The VP1 nucleotide sequence of 4 strains isolated from Shenzhen shared 87.8% - 92.0% identity with genogroup C, while the homogeneity of the VP4 nucleotide sequence was between 85.9% - 87.4%. The homogeneity of the VP1 nucleotide sequence with genotypes A and B was between 81.9% - 84.2% and was 80.6% - 85.0% with VP4. Among the 4 strains, the homogeneity of the VP1 nucleotide sequence was between 94.1% - 99.8% and was 100% with VP4 which formed a small group and could denominate EV71 genetype C4.

CONCLUSION: Similar results were obtained from phylogenetic analysis of EV71 based on VP1 and VP4 nucleotide sequence. The four EV71 strains causing epidemic in Shenzhen could be named as C4 subgroups.

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