Expression of cardiac function genes in adult stem cells is increased by treatment with nitric oxide agents

Carmen K Rebelatto, Alessandra M Aguiar, Alexandra C Senegaglia, Carlos M Aita, Paula Hansen, Fabiane Barchiki, Crisciele Kuligovski, Márcia Olandoski, José A Moutinho, Bruno Dallagiovanna, Samuel Goldenberg, Paulo S Brofman, Lia S Nakao, Alejandro Correa
Biochemical and Biophysical Research Communications 2009 January 16, 378 (3): 456-61
Mesenchymal stem cells (MSCs) have received special attention for cardiomyoplasty because several studies have shown that they differentiate into cardiomyocytes both in vitro and in vivo. Nitric oxide (NO) is a free radical signaling molecule that regulates several differentiation processes including cardiomyogenesis. Here, we report an investigation of the effects of two NO agents (SNAP and DEA/NO), able to activate both cGMP-dependent and -independent pathways, on the cardiomyogenic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived stem cells (ADSCs). The cells were isolated, cultured and treated with NO agents. Cardiac- and muscle-specific gene expression was analyzed by indirect immunofluorescence, flow cytometry, RT-PCR and real-time PCR. We found that untreated (control) ADSCs and BM-MSCs expressed some muscle markers and NO-derived intermediates induce an increased expression of some cardiac function genes in BM-MSCs and ADSCs. Moreover, NO agents considerably increased the pro-angiogenic potential mostly of BM-MSCs as determined by VEGF mRNA levels.

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