Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa

Michael A Palladino, Michael A Savarese, Jessica L Chapman, Mary-Katherine Dughi, Daniel Plaska
American Journal of Reproductive Immunology: AJRI 2008, 60 (6): 541-55

PROBLEM: Toll-like receptors (TLRs) are a family of innate immunity receptors that are essential for detecting invading pathogens. Objectives of this study were to identify epididymal cell types expressing TLRs and to determine if TLRs are present on spermatozoa.

METHOD OF STUDY: Immunohistochemical analysis of paraffin-embedded sections from regions of the rat epididymis was used to identify epididymal cell types expressing TLRs. Immunoblot analysis and immunofluorescence were used to detect TLRs on spermatozoa.

RESULTS: TLRs 1-9 and 11 are abundantly expressed by the epididymal epithelium in most regions of the duct with the exception of clear cells of the cauda which do not express TLRs 5-7 or 11. TLRs were detected on epididymal spermatozoa and several TLRs showed regional distributions patterns suggesting modification during epididymal transit.

CONCLUSION: The abundance of TLR family members in the epididymal epithelium and on spermatozoa provides strong evidence that TLRs play important roles in innate immunity of the male reproductive tract.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"