Rapid DNA multi-analyte immunoassay on a magneto-resistance biosensor

M Koets, T van der Wijk, J T W M van Eemeren, A van Amerongen, M W J Prins
Biosensors & Bioelectronics 2009 March 15, 24 (7): 1893-8
We present the rapid and sensitive detection of amplified DNA on a giant magneto-resistance sensor using superparamagnetic particles as a detection label. The one-step assay is performed on an integrated and miniaturized detection platform suitable for application into point-of-care devices. A double-tagged PCR amplification product of the LamB gene of the Escherichia coli bacterium was used to investigate binding kinetics of the assay. We applied magnetic actuation to concentrate the target-particle complexes at the sensor surface and to remove unbound particles from the sensor surface. We achieved biological dose-response curves detecting 4-250pM amplicon concentrations in a one-step format in total assay times of less than 3min. Using various tag-antibody combinations specific for one of the individual genes, multi-analyte detection is shown of several antibiotic resistance genes of the food pathogen Salmonella.

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