[Identification of the hemolytic transfusion reaction caused by lewis antibody using serological and molecular biological methods]

Xiao-Zhen Hong, Xian-Guo Xu, Fa-Ming Zhu, Kai-Rong Ma, Li-Xing Yan
Zhongguo Shi Yan Xue Ye Xue za Zhi 2008, 16 (5): 1192-5
To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le (a-b-) phenotype cells, patient phenotype was identified by using anti-Le(a) and anti-Le(b) blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly. The results showed that both IgM anti-Le(a) and anti-Le(b) antibodies were detected in patient's serum. Red cells was typed as Le (a-b-) phenotype and the FUT3 genotype was homozygote for non-functional le(59, 508) alleles. In conclusion, anti-Le(b) antibody can result in hemolytic transfusion reaction, FUT3 gene is homozygous for le(59, 508) allele resulting in Le (a-b-) phenotype.

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