Red cell alloimmunization in a transfused patient population: a study from a tertiary care hospital in north India

Beenu Thakral, Karan Saluja, Ratti Ram Sharma, Neelam Marwaha
Hematology (Amsterdam, Netherlands) 2008, 13 (5): 313-8

BACKGROUND: Red blood cell (RBC) alloimmunization results from genetic disparity of RBC antigens between donor and recipients. There is a paucity of data on the incidence of RBC alloimmunization in transfused recipients from the region studied, as pre-transfusion antibody screening is not routinely performed.

AIM: To assess the incidence of RBC alloimmunization in a transfused patient population at a tertiary care hospital in north India.

MATERIALS AND METHODS: Antibody screening was carried out in 531 multi-transfused patients prior to crossmatching with a commercially available three-cell panel (Diacell; Diamed AG, Cressier-sur-Morat, Switzerland) by the tube method, using a saline indirect antiglobulin test. Antibody screen-positive samples were analyzed for the specificity of the alloantibody with an 11-cell identification panel (Diapanel; Diamed AG). Antigen-negative crossmatch-compatible blood was transfused if the antibody was clinically significant, whereas for clinically insignificant antibodies, crossmatch-compatible blood at anti-human globulin phase was issued for transfusion.

RESULTS: The overall incidence of RBC alloimmunization in transfused patients was 3.4% (18/531), with anti-c being the most common specificity (38.8%), followed by anti-E (22.2%), anti-M (11.1%), anti-Le(a) (11.1%), anti-D (5.6%), anti-Jk(a) (5.6%) and anti-Le(b) (5.6%). The highest incidence of alloimmunization was observed in gastroenterology patients (4.5%), followed by hematology patients (3.5%). Of the antibodies detected, 16.7% (3/18) were clinically insignificant with Le(a), Le(b) and M specificities.

CONCLUSIONS: The majority of alloantibodies detected in the current study were clinically significant. Thus, pre-transfusion antibody screening on patients' samples prior to crossmatching needs to be initiated in India to ensure safe transfusion practice.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"