JOURNAL ARTICLE

Complete nucleotide sequence analysis of a Dengue-1 virus isolated on Easter Island, Chile

C Cáceres, V Yung, P Araya, J Tognarelli, E Villagra, L Vera, J Fernández
Archives of Virology 2008, 153 (10): 1967-70
18815724
Dengue-1 viruses responsible for the dengue fever outbreak in Easter Island in 2002 were isolated from acute-phase sera of dengue fever patients. In order to analyze the complete genome sequence, we designed primers to amplify contiguous segments across the entire sequence of the viral genome. RT-PCR products obtained were cloned, and complete nucleotide and deduced amino acid sequences were determined. This report constitutes the first complete genetic characterization of a DENV-1 isolate from Chile. Phylogenetic analysis shows that an Easter Island isolate is most closely related to Pacific DENV-1 genotype IV viruses.

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