JOURNAL ARTICLE

Comparative analysis of ESTs in response to drought stress in chickpea (C. arietinum L.)

Wen-Rui Gao, Xian-Sheng Wang, Qing-You Liu, Hui Peng, Chen Chen, Jian-Gui Li, Ju-Song Zhang, Song-Nian Hu, Hao Ma
Biochemical and Biophysical Research Communications 2008 November 21, 376 (3): 578-83
18804093
Chickpea (Cicer arietinum L.) is an important pulse crop grown mainly in the arid and semi-arid regions. To identify the water-stress-induced genes, two non-normalized cDNA libraries were constructed from the seedling leaves of a drought-tolerant chickpea cultivar under PEG-treated and -nontreated conditions. About 2500 clones from each library were selected randomly for sequencing analysis. Based on IDEG6 online software analysis, 92 genes were differentially expressed, and these genes were involved in diverse biological progresses, such as metabolism, transcription, signal transduction, protein synthesis and others. Most of the up-regulated genes were related to drought tolerance, and the down-regulated genes were mainly involved in photosynthesis. The differential expression patterns of five functional unigenes were confirmed by quantitative real-time PCR (qPCR). The results will help in understanding the molecular basis of drought tolerance in chickpea.

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