Co-circulations of two genotypes of dengue virus in 2006 out-break of dengue hemorrhagic fever in Karachi, Pakistan

E Khan, R Hasan, V Mehraj, A Nasir, J Siddiqui, R Hewson
Journal of Clinical Virology 2008, 43 (2): 176-9

BACKGROUND: The status of dengue genotypes involved in the recent epidemic out-breaks in Pakistan is not well defined.

OBJECTIVES: We sought to analyze the predominant genotype responsible for the most severe and largest out-break of dengue hemorrhagic fever (DHF) that hit Karachi in 2006.

STUDY DESIGN: Retrospective analysis of stored serum samples for dengue virus genotype by multiplex RT-PCR, anti-dengue IgM, IgG and review of clinical charts of patients admitted to Aga Khan University Hospital.

RESULTS: Viral RNA detection of 250 patients revealed positive results in 185 (74.0%) samples. DEN-2 was predominant genotype (n=104, 56.2%) Dengue specific antibodies were detected in 139 samples of which 81 were classified as primary cases. DEN-2 accounted for these. Within secondary cases, 63.2% were due to DEN-2 (total 57), the rest were positive for DEN-3. DHF (p=0.064) and abdominal pain (p=0.059) were more frequently associated with DEN-2 as compared to DEN-3. None of the samples were positive for DEN-1 or DEN-4.

CONCLUSION: Co-circulation of DEN-2 and DEN-3 was responsible for the 2006 out-break in Karachi. Primary and secondary cases were seen in both groups. Cases with DHF showed marginal association with DEN-2. Introduction of a new serotype (DEN-3) and or a genotypic shift of endemic serotype (DEN-2) are the probable factors for the recent out-break of DHF in this region.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Trending Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"