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English Abstract
Journal Article
Research Support, Non-U.S. Gov't
[Effect of human semaphorin-3F gene transient transfection on the proliferation of tongue carcinoma cells].
Zhonghua Kou Qiang Yi Xue za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology 2007 December
OBJECTIVE: To investigate the effect of semaphorin-3F (SEMA-3F) gene transient transfection on the proliferation of Tca8113 tongue carcinoma cells.
METHODS: After construction of a full-length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by Lipofectamine 2000. The expression of SEMA-3F gene was detected by RT-PCR The differences of the expression in the transfected cell groups, empty vector groups and un-transfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium (MTT) enzymatic labeling technique. Cell cycle were assayed by flow cytometer (FCM).
RESULTS: The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased.
CONCLUSIONS: SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.
METHODS: After construction of a full-length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by Lipofectamine 2000. The expression of SEMA-3F gene was detected by RT-PCR The differences of the expression in the transfected cell groups, empty vector groups and un-transfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium (MTT) enzymatic labeling technique. Cell cycle were assayed by flow cytometer (FCM).
RESULTS: The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased.
CONCLUSIONS: SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.
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