JOURNAL ARTICLE

A chemosensing ensemble for the detection of cysteine based on the inner filter effect using a rhodamine B spirolactam

Xiao-Feng Yang, Ping Liu, Liping Wang, Minglei Zhao
Journal of Fluorescence 2008, 18 (2): 453-9
18064543
A fluorescent chemosensing ensemble for the detection of cysteine is designed based on the fluorescence inner filter effect. The method employs the coordination of Cu(2+) ion with salicylaldehyde rhodamine B hydrazone (I), a colorless and non-fluorescent rhodamine B spirolactam derivative to form I-Cu(II), a pink color but weakly fluorescent complex. When rhodamine B was introduced to the I-Cu(II) complex solution, the fluorescence signal of rhodamine B is dramatically decreased because of the fluorescence inner filter effect (IFE). Upon adding cysteine to the above solution, it can complex preferentially to Cu(2+) compared to I, and the I-Cu(II) complex dissociates, which thus decreases the fluorescence IFE of the solution, and in turn leading to the fluorescence increase of the chemosensing system. Based on the above mechanism, a fluorescent chemosensing ensemble for cysteine is developed. The fluorescence increase is linearly with cysteine concentration up to 10.0 mu mol L(-1), with a detection limit of 1.4 x 10(-7) mol L(-1) (3sigma). The optimal conditions of the proposed method were studied and the selectivity of the proposed method was investigated in this paper.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
18064543
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"