JOURNAL ARTICLE

Compensative shuttling of merlin to phosphorylation on serine 518 in vestibular schwannoma

Jingrong Lü, Jing Zou, Hao Wu, Lihui Cai
Laryngoscope 2008, 118 (1): 169-74
17989580

BACKGROUND: Vestibular schwannoma (VS) is a benign tumor with malignant biological consequence because of its special location, and its occurrence is highly related to merlin. Cell culture studies have demonstrated that merlin acts as a molecular linker between the cytoskeleton and specific membrane proteins and is linked to cell cycle control. Therefore, we sought to detect the expression of endogenous merlin and its subcellular distribution using cyclin D1 as a cell cycle marker in VS.

METHODS: To confirm endogenous merlin protein expression in abiogenous VS cells, in situ hybridization, immunohistochemistry, and Western blot was performed in tumor tissues surgically resected from patients. Merlin's subcellular distribution linked cell cycle in VS was analyzed and compared with cyclin D1 expression evaluated with confocal microscopy.

RESULTS: We found that merlin appeared in 98% of the VS tissue samples, with a mean cellular positivity of 46.66 +/- 5.75%. Merlin is inversely correlated with cyclin D1 in regard to subcellular localization. Merlin locates in the cytoplasm during G0/G1 phase, moves to the nucleus at S phase, and accumulates in the cytoplasm at S phase after phosphorylation on serine 518 (S518).

CONCLUSION: In line with the pathologic characteristic of VS as a benign tumor, merlin compensates for the malignant effect of S518 phosphorylation by limiting the loss of contact inhibition of growth and controlling proliferation. S518 phosphorylation can trigger re-entry into the cell cycle, failing to control transcription because of mislocalization and an inability to interact with the cytoskeleton.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
17989580
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"