[Effect of isoflavones in regulating the transcription of target genes through estrogen receptors].

OBJECTIVE: To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes.

METHODS: In this study, we used ERalpha or ERbeta over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation.

RESULTS: Our results showed that both genistein and daidzein could activate ERE receptor gene through ERalpha and ERbeta, and these effects could be blocked by ICI 182780.

CONCLUSION: Both genistein and daidzein can mimic estrogen's effect to activate the transcription of target genes through binding to the ERs.