Quantitative and qualitative analysis of microorganisms in an assisted reproductive technology facility

Jason L Herlong, Ken Reubish, H Lee Higdon, William R Boone
Fertility and Sterility 2008, 89 (4): 847-53

OBJECTIVE: To perform a quantitative and qualitative analysis of the environmental microorganisms associated with two assisted reproductive technology (ART) laboratories.

DESIGN: Prospective descriptive study.

SETTING: Two ART laboratories at Greenville Hospital System (GHS) University Medical Center in Greenville, South Carolina.



MAIN OUTCOME MEASURE(S): Quantitative and qualitative analysis of colony-forming units (CFU; bacteria and fungi) and particles from an embryology clean room and an andrology laboratory.

RESULT(S): Within these laboratories, three of the sites sampled differed significantly in the mean number of CFU per plate. These sites included external laboratory surfaces, laboratory room air, and air within cell-culture incubators. The mean number of viable CFU per cubic meter of laboratory room air differed significantly between the two laboratories. A total of 23 unique microbial species were isolated from the andrology laboratory, whereas 10 unique species were isolated from the embryology clean room.

CONCLUSION(S): The data herein suggest that the increased positive control measures used in the embryology clean room are effective in minimizing environmental biologic contamination.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"