Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier.

A novel psychrotolerant, Gram-positive, yellow-pigmented, aerobic bacterium, strain 0549(T), was isolated from the China No. 1 glacier. The cells of the isolate were catalase-positive, motile, irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain 0549(T) was able to grow at 4-27 degrees C, with optimum growth occurring at 20-22 degrees C. The major fatty acids were anteiso-C(15 : 0), anteiso-C(15 : 1), iso-C(16 : 0) and anteiso-C(17 : 0). The genomic DNA G+C content was 67 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 0549(T) belonged to the genus Cryobacterium and exhibited 96.5 % similarity to Cryobacterium psychrophilum JCM 1463(T). On the basis of the phenotypic, chemotaxonomic and phylogenetic properties and the DNA-DNA relatedness data, strain 0549(T) represents a novel species of the genus Cryobacterium, for which the name Cryobacterium psychrotolerans is proposed. The type strain is 0549(T) (=CGMCC 1.5382(T)=JCM 13925(T)).