We have located links that may give you full text access.
ENGLISH ABSTRACT
JOURNAL ARTICLE
[Ad. mda-7/IL-24 construction and expression in infected drug resistant cell line of human ovarian cancer].
Sichuan da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition 2007 January
OBJECTIVE: To construct the recombinant Ad. mda-7/IL-24 using Adeasy 1 system.
METHODS: The mda-7/IL-24 DNA sequence was, by PCR, amplified from the plasmid pREP4-mda-7 and sub-cloned into the shuttle vector pAdTrack CMV. The resultant plasmid and pAdEasy 1 were used to co-transfer into the E. coli BJ5183 cells to undergo homologous recombination. The linearized recombinant plasmid DNA was transferred into 293 cells. Then the ovarian cancer drug resistant cell line OVCAR-8/TR was infected by the recombinant adenovirus, in which the expression of MDA-7/IL-24 protein was detected by Western-blot analysis.
RESULTS: The recombinant Ad. mda-7/IL-24 was constructed successfully and approved by sequence analysis, electrophoresis and expression of MDA-7/IL-24 protein. All OVCAR-8/TR cells had been infected under concentration of Ad. mda-7/IL-24 was 10 microL. OVCAR-8/TR cell had the objective protein expression after infected.
CONCLUSION: The recombinant adenovirus Ad. mda-7/IL-24 is successfully constructed, which lays a foundation for studying mda-7/IL-24 gene in the field of ovarian cancer drug resistant.
METHODS: The mda-7/IL-24 DNA sequence was, by PCR, amplified from the plasmid pREP4-mda-7 and sub-cloned into the shuttle vector pAdTrack CMV. The resultant plasmid and pAdEasy 1 were used to co-transfer into the E. coli BJ5183 cells to undergo homologous recombination. The linearized recombinant plasmid DNA was transferred into 293 cells. Then the ovarian cancer drug resistant cell line OVCAR-8/TR was infected by the recombinant adenovirus, in which the expression of MDA-7/IL-24 protein was detected by Western-blot analysis.
RESULTS: The recombinant Ad. mda-7/IL-24 was constructed successfully and approved by sequence analysis, electrophoresis and expression of MDA-7/IL-24 protein. All OVCAR-8/TR cells had been infected under concentration of Ad. mda-7/IL-24 was 10 microL. OVCAR-8/TR cell had the objective protein expression after infected.
CONCLUSION: The recombinant adenovirus Ad. mda-7/IL-24 is successfully constructed, which lays a foundation for studying mda-7/IL-24 gene in the field of ovarian cancer drug resistant.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app