Detection of Rickettsia rickettsii and Rickettsia sp. in blood clots in 24 patients from different municipalities of the State of Sao Paulo, Brazil

Flávia Sousa Gehrke, Elvira Maria Mendes do Nascimento, Eliana Rodrigues de Souza, Silvia Colombo, Luiz Jacintho da Silva, Teresinha Tizu Sato Schumaker
Annals of the New York Academy of Sciences 2006, 1078: 260-2
The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the gltA, ompA, and ompB genes. Sequence analysis was carried out using an automatic sequencer with Bioedit software. Seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced.

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