Add like
Add dislike
Add to saved papers

Japonica rice varieties (Oryza sativa, Nipponbare, and others).

Agrobacterium-mediated transformation of rice is now used in many laboratories worldwide. Several protocols have been developed and fine-tuned for particular genotypes, including commercial genotypes, making use of either mature seeds or immature embryos as target tissue for Agrobacterium infection. In this chapter, we describe a rapid and user-friendly protocol based on mature seeds that can deliver transgenic rice plant-lets within 2 mo. The protocol described is based on the use of the nptII selectable marker gene. The tissue culture steps rely on Agrobacterium infection of whole young seedlings and on the induction and proliferation of highly embryogenic tissue. Importantly, we have validated the robustness and reliability of this protocol at a high-throughput scale for several japonica genotypes. We also provide some key features that can be further explored for the fine-tuning of this transformation protocol for any other genotype with a particular emphasis on the importance of tissue handling and subculture sequence.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app