JOURNAL ARTICLE

Failure of shortening and inversion of the perinatal gubernaculum in the cryptorchid long-evans orl rat

Julia S Barthold, Xiaoli Si, Deborah Stabley, Katia Sol-Church, Liam Campion, Suzanne M McCahan
Journal of Urology 2006, 176 (4): 1612-7
16952701

PURPOSE: Failure of testicular descent occurs in about 65% of spontaneously cryptorchid Long-Evans orl rats. Development of the fetal gubernaculum is dependent on expression of leucine rich G protein coupled receptor (also known as G protein coupled receptor affecting testicular descent) and its ligand, insulin-like 3. We studied testicular descent and mRNA expression of insulin-like 3 and leucine rich G protein coupled receptor in Long-Evans orl and Long-Evans wild-type rats during perinatal development.

MATERIALS AND METHODS: Long-Evans orl and Long-Evans wild-type males obtained at gestational days 18 to 21 and day of birth were preserved in RNAlater for at least 24 hours. The size and position of the testes, kidneys and gubernacula were determined by microdissection and image analysis. Leucine rich G protein coupled receptor mRNA expression was analyzed in fetal gubernacula (gestational days 18 to 21) using real-time reverse transcriptase polymerase chain reaction with SYBR Green detection. Insulin-like 3 mRNA expression in fetal testis (gestational days 18 and 20) was determined by semiquantitative reverse transcriptase polymerase chain reaction.

RESULTS: Testicular position was similar in Long-Evans orl and Long-Evans wild-type fetal rats. However, gubernacula were narrow (p < 0.001) and elongated (p < or = 0.01) in Long-Evans orl compared to Long-Evans wild-type fetuses at all time points. Inversion of both gubernacula occurred by the day of birth in 45% of Long-Evans orl and 100% of Long-Evans wild-type males (p < 0.001). Leucine rich G protein coupled receptor mRNA expression decreased with age and, similar to insulin-like 3 mRNA, was not consistently different between strains.

CONCLUSIONS: These data suggest that the impaired shortening of the Long-Evans orl gubernaculum may interfere with the timing and quality of its inversion at birth, leading to failure of testicular descent in some newborn males. However, fetal testicular descent and the expression of leucine rich G protein coupled receptor and insulin-like 3 mRNA appear to be normal in this strain.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Trending Papers

Remove bar
Read by QxMD icon Read
16952701
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"