Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source

Elisa M P Ciapina, Walber C Melo, Lidia M M Santa Anna, Alexandre S Santos, Denise M G Freire, Nei Pereira Júnior
Applied Biochemistry and Biotechnology 2006, 129: 880-6
The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28 degrees C in a 1.5-L bioreactor using glycerol as carbon source. The bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. After 51 h of cultivation, 1.7 g/L of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mN/m, respectively, 67% of Emulsifying Index (E24), and 94% of oil removal were obtained. The use of glycerol rather than what happens with hydrophobic carbon source allowed the release of the biosurfactant, originally associated to the cell wall.

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