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ENGLISH ABSTRACT
JOURNAL ARTICLE
[Enhanced prokaryotic expression of a recombinant Lagurus lagurus zona pellucida subunit 3 with codon optimization].
Xi Bao Yu Fen Zi Mian Yi Xue za Zhi = Chinese Journal of Cellular and Molecular Immunology 2005 November
AIM: To improve prokaryotic expression level of Lagurus lagurus zona pellucida 3(LZP3) gene.
METHODS: The rare codons of LZP3 gene fragment were turned into the most frequently used codons in E. coli by overlap PCR. LZP3 gene and mutated LZP3 (LZP3m) gene were cloned into expression vector pGEX4T-1 respectively, and transformed into E. coli BL21(DE3).
RESULTS: The expression level of LZP3m was notably higher than that of LZP3.
CONCLUSION: The expression of LZP3 gene in E. coli could be successfully improved by using codon optimization.
METHODS: The rare codons of LZP3 gene fragment were turned into the most frequently used codons in E. coli by overlap PCR. LZP3 gene and mutated LZP3 (LZP3m) gene were cloned into expression vector pGEX4T-1 respectively, and transformed into E. coli BL21(DE3).
RESULTS: The expression level of LZP3m was notably higher than that of LZP3.
CONCLUSION: The expression of LZP3 gene in E. coli could be successfully improved by using codon optimization.
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