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English Abstract
Journal Article
[Construction of recombinant adenovirus expressing sCD40L-Ig].
Xi Bao Yu Fen Zi Mian Yi Xue za Zhi = Chinese Journal of Cellular and Molecular Immunology 2005 November
AIM: To construct adenovirus expressing secretory human CD40L-Ig (sCD40 ligand Ig fusion protein).
METHODS: The genes encoding the extracellular domain of human CD40L and IgG Fc were amplified with PCR and inserted into shuttle vector pAdTrack-CMV which then was transformed into E. coli pAdEasy-1-BJ5183 to produce recombinant Ad plasmid. The recombinant plasmid was digested with Pac I and transfected into 293 cells to generate recombinant adenovirus. The recombinant adenovirus was then used in mixed lymphocyte reaction (MLR) to test its function.
RESULTS: The recombinant adenovirus sCD40L-Ig was produced. It could inhibit the lymphocyte proliferation in MLR.
CONCLUSION: The sCD40L-Ig adenovirus is prepared successfully and its inhibition of MLR is confirmed.
METHODS: The genes encoding the extracellular domain of human CD40L and IgG Fc were amplified with PCR and inserted into shuttle vector pAdTrack-CMV which then was transformed into E. coli pAdEasy-1-BJ5183 to produce recombinant Ad plasmid. The recombinant plasmid was digested with Pac I and transfected into 293 cells to generate recombinant adenovirus. The recombinant adenovirus was then used in mixed lymphocyte reaction (MLR) to test its function.
RESULTS: The recombinant adenovirus sCD40L-Ig was produced. It could inhibit the lymphocyte proliferation in MLR.
CONCLUSION: The sCD40L-Ig adenovirus is prepared successfully and its inhibition of MLR is confirmed.
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