Carotid compression: investigation of cerebral autoregulative reserve in rats.

Easy-to-perform, reversible techniques to analyse cerebral autoregulation are still missing in animal research. The carotid compression technique has been established to investigate dynamic cerebral autoregulation in humans. Adapting the carotid compression technique, we compared data from the new application with that of a classical exsanguination method. Compressing the ipsilateral carotid artery with a non-traumatic clip device for 10s modulated cerebral perfusion pressure. After clip release, the peaking laser-Doppler flow velocity increase over the somatosensory cortex allowed calculation of the transient hyperaemic response ratio (THRR) in relation to baseline. Modulating blood-pressure levels maintenance of cerebral blood-flow velocity was compared with THRR responses. With decreasing blood-pressure levels, the THRR first increased (29+/-16% at 95+/-10 mmHg to 39+/-13% at 75+/-10 mmHg) before it returned to baseline values at 54+/-10 mmHg (27+/-14%). THRR significantly dropped to 11+/-12% at 34+/-11 mmHg when resting cerebral blood-flow velocity levels also started to decline. Based on the close correlation between blood-flow velocity levels and THRR responses, we have concluded that carotid compression is an alternative technique that can be used to assess cerebral autoregulation in rats. The technique allows less invasive and reversible testing of dynamic autoregulation to be performed, and the technique can easily be applied in conjunction with functional tests to potentially allow deeper insights into cerebral vasoregulative mechanisms.