JOURNAL ARTICLE

Giant cell formation through fusion of cells derived from a human giant cell tumor of tendon sheath

Masami Hosaka, Masahito Hatori, Richard Smith, Shoichi Kokubun
Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association 2004, 9 (6): 581-4
16228675
Although the mechanism of multinucleation in giant cell tumors of tendon sheath (GCTTS) remains unknown, two mechanisms have been proposed: one is cell fusion and the other amitotic division. The purpose of this study was to clarify the multinucleation process of cultured cells from GCTTS using an in vitro fluorescent cell membrane labeling technique. Cultured GCTTS cells obtained from a 7-year-old Japanese girl were divided into two groups, one for PKH-2 (green) staining and the other for PKH-26 (red) staining. After staining with the dyes, the cell populations were mixed and observed with a fluorescent microscope on the 4th and 14th days after mixing. On both the 4th and 14th days, the cultured GCTTS giant cells showed a mosaic of green and red colors, thus indicating cell membrane fusion. Images of double fluorescent labeled giant cells indicated cell fusion of mononucleated stromal cells that lead to multinucleated giant cells in these GCTTS cell cultures. These findings suggest that multinucleation in GCTTS results from the fusion of mononuclear stromal cells in vitro.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
16228675
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.