Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections

Maricel Vidal, Eileen Kruger, Claudia Durán, Rosanna Lagos, Myron Levine, Valeria Prado, Cecilia Toro, Roberto Vidal
Journal of Clinical Microbiology 2005, 43 (10): 5362-5
We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.

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