Effect of buformin and metformin on formation of advanced glycation end products by methylglyoxal

Tadashi Kiho, Motohiro Kato, Shigeyuki Usui, Kazuyuki Hirano
Clinica Chimica Acta; International Journal of Clinical Chemistry 2005, 358 (1): 139-45

BACKGROUND: The formation and accumulation of advanced glycation end products (AGE) in various tissues are known to be involved in the aging process and complications of long-term diabetes. Aminoguanidine as AGE inhibitors was first studied, and metformin as biguanide compounds have been reported to react with reactive dicarbonyl precursors such as methylglyoxal.

METHODS: We studied the effects of the biguanides of buformin and metformin on AGE formation by the methods of specific fluorescence, and enzyme-linked immunosorbent assay and a Western blot analysis using the anti-AGE antibody after incubating BSA or RNase with methylglyoxal.

RESULTS: Buformin is a more potent inhibitor of AGE formation than metformin, and suggests that the amino group of buformin trap the carbonyl group of methylglyoxal to suppress formation of AGE.

CONCLUSION: In addition to that of metformin, buformin may be clinically useful to prevent diabetic complications.

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