JOURNAL ARTICLE

Effects of hypothermia for a short period on histologic outcome and extracellular glutamate concentration during and after cardiac arrest in rats

Ken Takata, Yoshimasa Takeda, Tetsufumi Sato, Hideki Nakatsuka, Masataka Yokoyama, Kiyoshi Morita
Critical Care Medicine 2005, 33 (6): 1340-5
15942353

OBJECTIVE: To evaluate the therapeutic effects of hypothermia for a short period (20 mins, 31 degrees C) using a cardiac arrest model (5 mins) in rats.

DESIGN: Prospective animal study.

SETTING: Experimental laboratory in a university hospital.

SUBJECTS: Male Wistar rats (n = 42).

INTERVENTION: Direct current (DC) potential and extracellular glutamate concentrations (microdialysis) were monitored in the hippocampal region. Histologic observation was performed 7 days later.

MEASUREMENTS AND MAIN RESULTS: No animal died or showed severe complications as a result of hypothermia for a short period. In nontreated animals (group F), extracellular glutamate concentration simultaneously increased with the onset of membrane depolarization and continued to increase during the reperfusion period (maximum, 212% +/- 40% of the pre-ischemia level) until the onset of DC recovery. In animals in which hypothermia was initiated before the onset of ischemia (group A), extracellular glutamate concentration did not increase during the ischemia period. When hypothermia was initiated at the onset of resuscitation (group B), the glutamate concentration immediately decreased. In animals in which hypothermia was initiated at 4.9 +/- 1.3 mins (immediately after DC recovery, group C), 10 mins (group D), and 20 mins (group E) after the onset of resuscitation, changes in extracellular glutamate concentration were the same as those in nontreated animals. The percentage of injured neurons was significantly attenuated (compared with group F, 82% +/- 10%) when hypothermia was initiated before DC recovery (group A, 5% +/- 3%; group B, 29% +/- 22%) or immediately after DC recovery (group C, 58% +/- 18%, 9.9 +/- 1.3 mins after the onset of ischemia).

CONCLUSIONS: Hypothermia for a short period decreased glutamate concentration when it was initiated before DC recovery and attenuated neuronal damage when it was initiated before or immediately after DC recovery. The therapeutic time window for hypothermia for a short period is about 10 mins after the onset of ischemia.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Trending Papers

Remove bar
Read by QxMD icon Read
15942353
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.