Separation and identification of multicomponent mixture by thin-layer chromatography coupled with Fourier transform-infrared microscopy

Wenxuan He, Guangqiang Cheng, Fei Zao, Yingchun Lin, Jianming Huang, Robert Shanks
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy 2005, 61 (8): 1965-70
A fast and convenient method based on coupled thin-layer chromatography (TLC) with Fourier transform infrared (FTIR) microscopy has been established for separation and identification of multicomponent mixtures. In this study, the method was developed and consummated with more perfect TLC spots transferral process and consistent FTIR testing conditions. A newly developed technique, solid-phase extract (SPE) was introduced for sample pre-treatment instead of using traditional column chromatography. It is a new field for SPE that has already been widely applied in many other fields. It not only overcomes the backwards (low separation efficiency, time consuming and solvent consumption) of column chromatography but also makes it much easier to choose an optimum TLC sheet and to set suitable TLC loading. With all the above-mentioned modifications and supplements, the analytical method of coupled TLC with FTIR microscopy for separation and identification of multicomponent mixtures becomes more convenient and more efficient. In addition, a very complex sample (a die-cast release agent) was used as an example to demonstrate the technique.

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