[Platelet-rich plasma made by a modified method promotes proliferation of rat osteoblast and human osteoblast in vitro]

Yang Song, Yonglie Chao, Ping Gong
Chinese Journal of Reparative and Reconstructive Surgery 2005 March 15, 19 (3): 178-82

OBJECTIVE: To study the effect of serum rich in growth factors (SRGF) derived from platelet-rich plasma (PRP) on the biological function of human and rat osteoblast.

METHODS: PRP and platelet-poor plasma (PPP) obtained from healthy human and SD rat were activated by thrombin to get SRGF and serum poor in growth factors (SPGF). The level of TGF-beta1 and PDGF-AB in human-SRGF and SPGF were assayed by enzyme-linked immunoassay (ELISA). Rat and human osteoblast were cultured and identified. Rat osteoblasts were treated with 5% rat-SRGF, 5% rat-SPGF and serum-free F12 medium, respectively. And human osteoblast were treated with 5% human-SRGF, 5% human-SPGF and serum-free DMEM. Cellular mitogenic activity was evaluated by thiazolyl blue (MTT) colorimetric assay at 24, 48, 72 and 96 hours.

RESULTS: The level of TGF-beta1 in human-SRGF was 307.67 +/- 35.57 ng/ml, and that of PDGF-AB was 52.76 +/- 7.89 ng/ml. The proliferation of rat and human osteoblast were promoted after treated with rat-SRGF and human-SRGF, respectively. In rat osteoblast groups, there were significant differences in absorbency between rat-SPGF group and rat-SRGF group at 48 and 96 hours (P < 0.05). In human osteoblast groups, the differences between human-SPGF group and human-SRGF group were significant at 48, 72 and 96 hours (P < 0.05). The proliferation of these two kinds of osteoblasts almost stopped in serum-free medium, and the differences in absorbency, compared with other groups, were significant (P < 0.05).

CONCLUSION: High quality of PRP can be achieved by the improved method and SRGF is capable of up-regulating the proliferation of rat osteoblast and human osteoblast.

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